Ameliorative effects and histological assessment of active fractions of ethanolic fruits extract of Raphia hookeri on AlCl3 induced toxicity in rats
DOI:
https://doi.org/10.18203/2349-3933.ijam20240357Keywords:
Raphia hookeri, Liver function, Kidney function, Lipid profile, Histopathological assessmentAbstract
Background: Aluminium chloride is a widely distributed element with a well-established toxicity. The study aimed at evaluating ameliorative effects of active fractions and ethanolic fruits extracts of Raphia hookeri on AlCl3-induced toxicity in male rats. The objectives included; determination of liver and kidney function biomarkers, lipid profile, histopathological assessment of the organs.
Methods: A total of 110 healthy male rats weighing 180-200 g were grouped into 11 groups of 10 rats each. Group 1: Normal feed and water (normal control). Group 2: AlCl3 only. Group 3: 200 mg/kg b. w of vitamin C. Group 4 and 5: N-hexane fraction at 10 and 20 mg/kg b. w. Group 6 and 7: ethyl acetate fraction at 10 and 20 mg/kg b. w. Group 8 and 9: Aqueous fractions at 10 and 20 mg/kg b. w. Group10 and 11: Ethanol extract at doses of 200 mg/kg b. w and 400 mg/kg b. w. The treatment lasted for 21 days.
Results: Results revealed a significant (p˂0.05) decreased in the activities of ALT, AST, ALP, TB, DB and TP. It further revealed a significant (p˂0.05) decrease in urea, creatinine, sodium, potassium and chloride. Also, a significant (p˂0.05) decrease in CHOL, TG, HDL-C, LDL-C was observed. Histopathological assessment of the liver and kidney tissues corroborated the observed changes in enzymes activities.
Conclusions: The findings demonstrated ameliorative potentials of active fractions and ethanolic fruit extract of Raphia hookeri against hepatic and renal damage induced by AlCl3 in a dose and time dependent manner.
References
Al-Eisa R, Khouja H, Al-Nahari H. Turmeric (Curcuma Longa) protection against the liver toxicity caused by aluminum chloride (AlCl3) in Adult Male Rats. Int J Pharma Res Allied Sci. 2017;6(2):32-8.
Machado-Neves M, Dias FCR, de Almeida GD. The effect of aluminum on mitochondrial dysfunctions. In: Mitochondrial Intoxication. India: Academic Press; 2023;205-34.
Othman MS, Fareid MA, Abdel Hameed RS, Abdel Moneim AE. The Protective Effects of Melatonin on Aluminum-Induced Hepatotoxicity and Nephrotoxicity in Rats. Oxid Med Cell Longev. 2020;2020:7375136.
Al-Kahtani M, Abdel-Daim MM, Sayed AA, El-Kott A, Morsy K. Curcumin phytosome modulates aluminum-induced hepatotoxicity via regulation of antioxidant, Bcl-2, and caspase-3 in rats. Environ Sci Pollut Res Int. 2020;27(17):21977-85.
Alausa SK, Oyesiku OO, Aderibigbe JO, Akinola OS. Thermal properties of Calamus deërratus, Raphia hookeri and synthetic board in building design in Southwestern Nigeria. Afr J Plant Sci. 2011;5(4):281-3.
Checkouri E, Reignier F, Robert-Da Silva C, Meilhac O. Evaluation of Polyphenol Content and Antioxidant Capacity of Aqueous Extracts from Eight Medicinal Plants from Reunion Island: Protection against Oxidative Stress in Red Blood Cells and Preadipocytes. Antioxidants. 2020;9(10):959.
Akpan EJ, Usoh IF. Phytochemical screening and effect of aqueous root extract of Raphia hookeri (Raffia palm) on metabolic clearance rate of ethanol in rabbits. Biokemistry. 2004;16:37-42.
Guidelines for testing of chemicals-423, Documentation on Acute Oral toxicity and Acute class method Available at: http://www.oecd.org. Accessed on 20 May 2023.
Christijanti W, Mohamed MA, Ahmed MA, El Sayed RA. Molecular effects of Moringa leaf extract on insulin resistance and reproductive function in hyperinsulinemic male rats. J Diabetes Metab Disord. 2019;18(2):487-94.
Ezeonu CS, Yakubu OE, Nwokwu CD, Azubuike NC, Sallah YG. Effects of ethanolic extracts of leaf, seed and fruit of Datura metel L. on kidney function of male albino rats. J Tradit Complement Med. 2018;9(4):271-7.
Uhegbu FO, Imo C, Onwuegbuchulam CH. Lipid lowering, hypoglycemic and antioxidant activities of Chromolaena odorata (L) and Ageratum conyzoides (L) ethanolic leaf extracts in albino rats. J Med Plants Stud. 2011;4(2):155-9.
Ge M, Zhao H, Yan Y, Zhang T, Zeng J, Zhou W, Wang Y, Meng Q, Zhang C. Performance of electrolyte measurements assessed by a trueness verification program. Clin Chem Lab Med. 2016;54(8):1319-27.
Imo C, Arowora KA, Ezeonu CS, Yakubu OE, Nwokwu CD, Azubuike NC, Sallah YG. Effects of ethanolic extracts of leaf, seed and fruit of Datura metel L. on kidney function of male albino rats. J Tradit Complement Med. 2018;9(4):271-7.
Titford M. The long history of hematoxylin. Biotechnol Histochem. 2005;80(2): 73-80.
Mohammed AA. Renal Damage Mediated by Oxidative Stress in Mice Treated with Aluminium Chloride: Protective Effects of Taurine. J Biol Sci. 2010;10(7):584-95.
Krewski D, Yokel RA, Nieboer E, Borchelt D, Cohen J, Harry J, Kacew S, Lindsay J, Mahfouz AM, Rondeau V. Human health risk assessment for aluminium, aluminium oxide, and aluminium hydroxide. J Toxicol Environ Health B Crit Rev. 2007;10(1):1-269.
Hassan NM, Alhossary AA, Mu Y, Kwoh CK. Protein-Ligand Blind Docking Using QuickVina-W With Inter-Process Spatio-Temporal Integration. Sci Rep. 2017;7(1):15451.
Willhite CC, Karyakina NA, Yokel RA, Yenugadhati N, Wisniewski TM, Arnold IM, et al. Systematic review of potential health risks posed by pharmaceutical, occupational and consumer exposures to metallic and nanoscale aluminum, aluminum oxides, aluminum hydroxide and its soluble salts. Crit Rev Toxicol. 2014;44(4):1-80.
Al Dera HS. Protective effect of resveratrol against aluminum chloride induced nephrotoxicity in rats. Saudi Med J. 2006;37:369.
Ighodaro MO, Omale JO, Fadipe SO. Effects of piliostigma thonningii ethyl acetate leaf extract on aluminium-cum extract treated Wistar rats. Animal Res Int. 2012;9(2):1579-84.
Yakubu OE, Nwodo OFC, Abdulrahaman M, Uyeh LB. Effects of Vitex doniana leaf extract on aluminium-induced toxicity in male albino Wistar rats. J Applied Biol Biotechnol. 2016;4(5):37-40.
Burtis CA, Ashwood ER, Bruns DE. Tietz Fundamentals of Clinical Chemistry. 6th ed. New Delhi: Elsevier; 2008:322-9.
D'Abrosca B, Dellagreca M, Fiorentino A, Monaco P, Previtera L, Simonet A. Limoncella’ apple, an Italian apple cultivar: Phenolic and flavonoid contents and antioxidant activity. Food Chem. 2007;104(4):1333-7.
Newairy A, Salama A, Hussien H, Yousef M. Propolis alleviates aluminum induce lipid peroxidation and biochemical parameters in male rats. Food Chem Toxicol. 2009;47:1093-8.
Shati AA, Alamri SA. Role of saffron (Crocus sativus L.) and honey syrup on aluminum-induced hepatotoxicity. Saudi Med J. 2010;31(10):1106-13.
John J, Nampoothiri M, Kumar N, Mudgal J, Nampurath GK, Chamallamudi MR. Sesamol, a lipid lowering agent, ameliorates aluminium chloride induced behavioral and biochemical alterations in rats. Pharmacogn Mag. 2015;11(42):327-36.
Mardani S, Nasri H, Hajian S, Ahmadi A, Kazemi R, Rafieian-Kopaei M. Impact of Momordica charantia extract on kidney function and structure in mice. J Nephropathol. 2014;3(1):35-40.
Asgari SA, Mohammadi M. The role of intraprostatic inflammation in the acute urinary retention. Int J Prev Med. 2011;2(1):28-31.
Noah U, Udotong J. Protective potential of ethanol leaf extract of Vernonia amygdalina against aluminium chloride induced renal toxicity in male Wistar rats. J Med Plant Studies. 2010;8(3):39-43.
Akpanyung EO, Nwaokonko DU, Moses B, Ekpo MM. Evaluation of the protective effect of Moringa oleifera leaf extract against aluminium induced liver damage in male albino Wistar rats. Int J Sci. 2018;4(2):20-30.
Egbono FF, Mene AE, Nwiko KM. Liver Enzymes Functions Effect of Raphia Hookeri Fruit Pulp (Mesocarp) Extract in Male Wistar Rat‟s Model. Schol Int J Biochem. 2010;6(8):99-105.
