Published: 2017-07-20

Age and sex related serum changes in nitric oxide and its correlation with plasma lipid profile and lipid peroxidation in healthy ageing individuals

Rohit Sheth, Tanmay Bhat, Suchetha Kumari N., Harshini Devi Ullal


Background: The free radical theory of aging postulates that aging results from the accumulation of deleterious effects caused by free radicals, and the ability of an organism to cope with cellular damage induced by ROS plays an important role in determining organismal lifespan. GSH and SOD functions by donating the proton and in scavenging the superoxide radicals, thereby protecting the body against oxidative stress by scavenging the free radicals produced in the body. Glutathione peroxidase also have similar function, it reduce lipid hydroperoxides to their corresponding alcohols and free hydrogen peroxide to water.

Methods: Hundred healthy adults from staff and student community under the age group of 20 to 60 years were recruited. Approximately 5ml blood sample was collected and used for the analysis of lipid profile, MDA and antioxidant status using standard protocol.

Results: The lipid profile, MDA and antioxidant status were measured and compared with that of serum nitric oxide levels of 100 healthy individuals of the age 20-60 yrs. Men aged 20-29 years showed significantly higher NO levels compared to corresponding women. There is a significant reduction in total antioxidant capacity in elderly people. With the decrease in NO there is increase in MDA is observed.

Conclusions: There is a significant reduction in total antioxidant capacity in elderly people. This reduction in antioxidant capacity implies a defect in antioxidant system, may be due to a reduction in individual antioxidant or may be caused by a non-equilibrium or poor cooperation between them.


Nitric oxide, Lipid peroxidation, Plasma lipid profile, Total antioxidant capacity

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Bowen RL, Atwood CS. Living and dying for sex. A theory of ageing based on the modulation of cell cycle signalling by reproductive hormones. Gerontol. 2004;50(5):265-90.

Aging and Gerontology Glossary. Retrieved 2011-02-26.

Stryer, Lubert. Biochem. 4th Edition. W.H. Freeman and Company; 1995;732.

Wink DA, Kasprzak KS. DNA deaminating ability and genotoxicity of nitric oxide and its progenitors. Sci. 1991;254(5034):1001

Nguyen T, Brunson D, Crespi CL, Penman BW, Wishnok JS, Tannenbaum SR. DNA damage and mutation in human cells exposed to nitric oxide in vitro. Proc Natl Acad Sci. USA. 1992;89(7):3030-4.

Li CQ, Pang B, Kiziltepe T, Trudel LJ, Engelward BP, Dedon PC, et al. Threshold effects of nitric oxide-induced toxicity and cellular responses in wild-type and p53-null human lymphoblastoid cells. Chem Res Toxicol. 2006;19(3):399-406.

Neil Hogg, B Kalyanaraman. Nitric oxide and lipid peroxidation. Biochimica et Biophysica Acta (BBA)-Bioenergetics. 1999;1411(2-3)5:378-84.

Di Massimo C, Lo Presti R, Corbacelli C, Pompei A, Scarpelli P, De Amicis D, Caimi G, et al. Impairment of plasma nitric oxide availability in senescent healthy individuals: apparent involvement of extracellular superoxide dismutase activity. Clin Hemorheol Microcirc. 2006;35(1-2):231-7.

Peinado MA, López-Ramos JC, Camacho MV, Molina FJ, et al. Age and sex-related serum changes in nitric oxide: correlations with serological markers. Int J Cardiol. 2007;121(1):88-90.

DeNino WF, Tchernof A, Dionne IJ, Toth MJ, Ades PA, Sites CK, Poehlman ET. Contribution of abdominal adiposity to age-related differences in insulin sensitivity and plasma lipids in healthy nonobese women. Diabetes care. 2001;24(5):925-32.

Jensen J, Nilas L, Christiansen C. Influence of menopause on serum lipids and lipoproteins. Maturitas. 1990;12(4):321-31.

L. Hayflick. How and why we age. Experimental Gerontol. 1998;33 (7-8):639-653.

Kirkwood TBL. Understanding the odd science of aging. Cell. 2005;120(4):437-47.

Harman D. Aging: a theory based on free radical and radiation chemistry. J Gerontol. 1996;11 (3):298-300.

Ghasemi A, et al. Serum nitric oxide metabolite levels in a general healthy population: relation to sex and age. Life Sci. 2008;83(9-10):326-31.

Chen Z, Niki E. Two faces of lipid peroxidation products: the Yin and Yang principles of oxidative stress. J Experimental and Integrative Medicine; 2011;1(4):215-9.

Eqbal M.A. Dauqan, et al. Natural antioxidants, lipid profile, lipid peroxidation, antioxidant enzymes of different vegetable oils. Advance J Food Sci Tech. 2011;3(4):308-16.

F Nabatchin, Khaghani S, Bagherian R, Miri R, Mahmoodi M, Pasalar P. The relation between oxidative stress and onset of coronary artery disease. Med J Islamic Rep Ir. 2004;18(2):101-5.

Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol. 1978;52:302-10.

Ghasemi A, Zahediasl S, Azizi F. Reference values for serum nitric oxide metabolites in an adult population. Clin Biochem. 2010;43(1):89-94.