DOI: http://dx.doi.org/10.18203/2349-3933.ijam20181071

Serum calcium and phosphorus levels: a marker of disease activity in senile cataract patients

Smita A. Deokar, Pooja S. K. Rai, Anita B. Rai, . Sudarshan, Shimi Sundharan, Amruta A. Bakshi

Abstract


Background: The present study was aimed to study alterations in serum levels of calcium and phosphorus levels in senile cataract patients.

Methods: 25 senile cataract patients in age group of 50 to 80 years and 25 control group were included in the study. Serum Calcium and Phosphorus levels were determined by Orthocresolphthalein, Fiske SubbaRow method respectively

Results: Significantly increased levels of serum calcium in cataract patients (11.58±1.65 mg/dl) were found as compared to controls (8.53±1.45mg/dl) (p<0.0001). Serum phosphorus concentration in cataract patients (5.28±0.46 mg/dl) were significantly increased when compared to controls (3.02±1.23mg/dl) (p<0.0001).

Conclusions: Presence of G-protein receptors in lens leads to the release of intracellular calcium.  As total calcium in the lens increases, we hypothesize that higher intercellular calcium concentrations, coupled with decreased Ca2+-ATPase activity and greater membrane permeability could lead to elevated free intracellular calcium levels causing cataract. So, abnormal elevation of serum calcium and phosphorus can be used as a marker for prevention of age-related human cataract.


Keywords


Calcium, Phosphorus, Senile cataract

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References


Zampighi GA. The lens. In: Fischbarg J, ed. Advances in Organ Biology. London: Elsevier;2006;10:149-79.

Benedek GB. Theory of transparency of the eye. Appl Optics. 1971;10:459-73.

Duncan G, Wormstone IM, Davies PE. The aging human lens: structure, growth, and physiological behaviour. Br J Ophthalmol. 1997;81(10):818-23.

Brian G, Taylor H. Cataract blindness: challenges for the 21st century. Bull World Health Organization. 2001 Jan;79(3):249-56.

Zigler Jr JS, Hess HH. Cataracts in the Royal College of Surgeons rat: evidence for initiation by lipid peroxidation products. Exp Eye Res. 1985;41(1):67-76.

Harding JJ, Crabbe MJC. The lens: development, proteins, metabolism and cataract. In: Harding J, ed. The Eye. London: Academic Press;1984:207-492.

Danysh BP, Duncan MK. The lens capsule. Exp Eye Res. 2009;88:151-64.

Harding J. The normal lens. In: Harding J, ed. Cataract: Biochemistry, Epidemiology and Pharmacology. London: Chapman and Hall. 1991:1-63.

Bhat SP. The ocular lens epithelium. Biosci Rep. 2001;21:537-63.

Sugiyama Y, Prescott AR, Tholozan FM, Ohno S, Quinlan RA. Expression and localisation of apical junctional complex proteins in lens epithelial cells. Exp Eye Res. 2008 Jul;87(1):64-70.

Davies MJ, Truscott RJ. Photo-oxidation of proteins and its role in cataractogenesis. J Photochem Photobiol Biol. 2001;63:114-25.

Pierscionek BK, Augusteyn RC. Structure/function relationship between optics and biochemistry of the eye lens. Lens Eye Toxic Res. 1991;8:229-43.

Augusteyn R. Growth of the human eye lens. Mol Vis. 2007;13:252-7.

Sharma K, Santhoshkumar P. Lens aging: effects of crystallins. Biochim Biophysica Acta. 2009;1790:1095-108.

Umapathy A, Donaldson P, Lim J. Antioxidant delivery pathways in the anterior eye. BioMed Res Int. 2013;1-10.

Duncan G, Wormstone IM. Calcium cell signalling and cataract: role of the endoplasmic reticulum. Eye. 1999 May;13(3b):480.

Paterson CA, Zeng J, Husseini Z, Borchman D, Delamere NA, Garland D et al. Calcium ATPase activity and membrane structure in clear and cataractous human lenses. Current Eye Res. 1997;16(4):333-8.

Li W, Calvin HI, David LL, Wu K, McCormack AL, Zhu GP et al. Altered patterns of phosphorylation in cultured mouse lenses during development of buthionine sulfoximine cataracts. Exp Eye res. 2002 Sep;75(3):335-46.

Duncan G, Jacob TJ. Calcium and the physiology of cataract. InCiba Foundation Symposium. 106-Human Cataract Formation John Wiley & Sons, Ltd;2008:132-162.

Sanderson J, Duncan G. pCMPS-induced changes in lens membrane permeability and transparency. Invest. Ophthalmol Vis Sci. 1993 Jul;34(8):2518-25.

Delamere NA, Paterson CA. Hypocalcaemic cataract: mechanisms of cataract formation in the Human Lens. 1981:219-36.

Duncan G, Hightower KR, Gandolfi SA, Tomlinson J, Maraini G. Human lens membrane permeability increases with age. Invest Ophthalmol Vis Sci. 1989;30:1855-9.

Marcantonio JM, Duncan G, Rink H. Calcium-induced opacification and loss of protein in the organ-cultured bovine lens. Exp Eye Res. 1986;42:617-30.

Tenenhouse HS. Regulation of phosphorus homeostasis by the type iia na/phosphate cotransporter. Annu Rev Nutr. 2005;11;25:197-214.

Stern J, Lewis WHP. The colorimetric estimation of calcium in serum with o-cresolphthalein complexone. Clin Chim Acta. 1957;2:576-80.

Fiske CH, SubbaRow YJ. The colorimetric determination of phosphorus. J Biol Chem. 1925;66:375.

Duncan G, Williams MR, Riach RA. Calcium, cell signaling and cataract. Prog Retinal Eye Res. 1994;12:623-51.

Deokar SA, Rai PS, Ingale PW, Rai AB, Bakshi AA. Study of serum sodium and potassium concentration in cataract patients. Int J Res Med Sci. 2014;2:592-4.

Clark JI, Mengel L, Bagg A, Benedik GB. Cortical opacity, Calcium concentration and fiber membrane structure. Exp Eye Res. 1980;31:399-410.

Zeng J, Borchman D, Paterson CA. ATPase activities of rabbit and bovine lens epithelial microsomes: a continuous fluorimetic assay study. Curr Eye Res. 1995;14:87-93.

Duncan G, Webb SF, Dawson AP, Bootman MD, Elliott AJ. Calcium regulation in tissue cultured human and bovine lens epithelial cells. Invest Ophthalmol Vis Sci. 1993;34:2835-42.

Bian L, Zeng J, Borchman D, Paterson CA. Plasma membrane calcium ATPase gene expression in bovine lens epithelium. Ophthalmic Res. 2000;32:100-5.

Gandolfi SA, Tomba CM, Maraini G. 86-Rb Efflux in normal and cataractous human lenses. Curr Eye Res. 1985;4:753-8.

Paterson CA, Zeng J, Hussenini Z, Borchman D, Delamere NA, Garland D, et al. Calcium ATPase activity and membrane structure in clear and cataractous human lenses. Curr Eye Res. 1997;16:333-8.

Delamere NA, Paterson CA, Borchman D, King KL, Cawood SC. Calcium transport, Ca-ATPase and lipid order in rabbit ocular lens membranes. Am J Physiol. 1991;260:C731-C737.

Bakas LS, Disalvo EA. Effect of asymmetric Ca2distribution on the bilayer properties of phosphatidylcholine-sonicated vesicles. Biochem Biophys Acta. 1989;979:352-60.